Ge akta purifier user manual

Ensure your akta system is operating at peakperformance by priming. A fraction collector that can gather up to 100 fractions either dropwise or at specified time intervals is provided with the akta basic fplc system. Contact your local ge healthcare representative for the most current information. Amersham biosciences ge fplc system cambridge scientific.

Ge healthcare data file 11003543 ac process chromatography aktaprocess aktaprocess is an automated liquid chromatography system built for process scaleup and largescale biopharmaceutical manufacturing fig 1. Laboratory equipment ge aktapure user manual 522 pages laboratory equipment ge akta pure operating instructions manual. Oct 16, 2019 akta purifier manual pdf about this manual. If any damage is found, document the damage, and contact your local ge healthcare representative. The ge aktaprime plus fplc system offers a lab scale, one step, flexible solution for liquid chromatography and protein purification needs. Data file 11003543 ac process chromatography aktaprocess. If the leakage persists, replace the assembly according to instructions in the pump p960 user manual. Loops of 10 150ml, termed superloops, are available from ge healthcare. Akta start is an easytouse chromatography system that automates manual purification procedures using for example hitrap columns. Get familiarized with its use and have its instruction manual handy. Ge pharmacia akta purifier 100 gmi trusted laboratory.

Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Akta avant is a preparative protein purification chromatography system designed for fast and secure development of scalable methods and processes. Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. Akta start protein purification system, ge healthcare vwr. Ge healthcare akta with the ability to purify virtually any biomolecule, akta protein purification systems can handle the simplest and the toughest of challenges. It offers an entry level chromatography solution for the preparative purification of proteins in lab scale.

Akta pure protein purification system ge healthcare life. Laboratory equipment ge imagequant las 4000 user manual. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Akta fplc is a fully automated liquid chromatography system designed for research. Akta avant user manual protein production and purification. For detailed information on installation, see aktaprime plus user manual. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin.

Akta chromatography systems have been a staple in protein purification for the past two decades and used throughout the globe. The tray and racks are inserted into the fraction collector with the ge mono. Ge akta purifier user manual download ge akta purifier user manual. Ge healthcare life sciences imagination at work akta laboratoryscale chromatography systems instrument management handbook 29010831 ge healthcare life sciences antibodypurification handbook antibody purification handbook 18103746 ge healthcare life sciences biacore assay handbook biacore assay handbook 29019400 ge healthcare life sciences. Centrifuge or filter the samples prior sample loading, unless columns.

It will help if they get w10 with some good dev support when it comes out. Follow the instructions given in the frac920 user manual. The ge akta purifier 10 is a flexible modular fast protein liquid chromatography system. Ge akta explorer amersham pharmacia certified fplc system. Laboratory equipment ge akta avant operating instructions manual. Akta avant cytiva, formerly ge healthcare life sciences. Jan 09, 2018 cytiva formerly ge healthcare life sciences 57,922 views 5. Ge akta start maintenance manual pdf download manualslib. Ensure that the hg lamp position and the filter is selected according. Sections 1 5 contain the necessary information for operating the instrument.

For further details see akta avant user manual and akta pure user manual. Check valve is clogged or damaged, causing leakage. Aktapurifier 10 cytiva, formerly ge healthcare life sciences. The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to. Unpacking all parts are either mounted on the system or located in the accessory kit box. Ge healthcare aktapurifier user guide important user information ce. Aktafplc is a fully automated liquid chromatography system designed for research. This manual describes the operation of the aktafplc system. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c i en c a m e r s h a m b io s c i e n c e akta system training guide explorer 100 10. Aktapurifier 10 100 a system pumps mixer 2 4 7 3 1 6 5 2 3 4 5 6 7 1 8 fraction collector frac950 conductivity monitor ph detector inline filter uv cond. System description, system maintenance and troubleshooting are also. Healthcare except for alterations described in this manual. File containing module user manuals and component instructions for aktafplc equipment pump p920 user manual monitor upc900 user manual. Aktapurifier user guide ge healthcare life sciences manualzz.

Akta labscale protein purification systems are designed for purification of biomolecules, providing speed, performance, and flexibility in research and process development. Please see the aktadesign optimal configuration user manual for the recommended flow rates. It also contains an overview of the safety instructions. Ge healthcare life sciences offers a wide range of prepacked columns, such as hitrap columns, to help you with protein purification. This high flow rate is necessary when working with. Laboratory equipment ge aktapure user manual 522 pages. Ge akta explorer amersham pharmacia certified fplc system gmi. Chromatographic techniques at flow rates and pressures compatible with modern separation media are used to produce highcaliber solutions.

Jan 25, 2017 cytiva formerly ge healthcare life sciences 8,742 views 2. The operating instructions provide you with the instructions needed to handle fraction. Aug 12, 2019 akta purifier manual pdf about this manual. Bioscisolutions repairs your akta, biacore, and agilent. Contents monitor upc900 user manual 18112555 edition ag vii. Akta fplc system operators manual marshall scientific by. Apr 19, 2020 akta purifier manual pdf about this manual. Jun 20, 2019 akta purifier manual pdf about this manual. Evaluating the uv response calculate the uv response ratios in the following way. Loosen the lock knob 3 akta avant user manual 29035184 ae. Akta laboratoryscale chromatography systems handbook. Ge healthcare uk limited amersham place little chalfont buckinghamshire, hp7 9na uk ge healthcare europe, gmbh munzinger strasse 5 d79111 freiburg germany. The proven design has been verified during development and can be userconfigured to meet specific process demands.

Ge healthcare akta high flow kit akta user kit liquid. Documents document view page ge pharmacia biotech akta. Fplc standard operating procedure akta purifier system page page 1 of 9 fplc standard operating procedure akta system ge note. Akta systems use intelligent unicorn system control software to combine simplicity with power in protein purification. Its well suited to general purification tasks like gel filtration of recombinant dna, ion exchange, buffer exchange and hydrophobic interaction chromatography, as well as affinity chromatography. The user must read and understand the safety instructions in this manual. Priming system and sample pumps for aktaexplorer, aktapurifier and aktafplc. A copy of these terms and conditions is available on request.

The ge akta explorer chromatography systems, formally from amersham pharmacia biotech offer fast and reliable solutions for developing and optimizing any biomolecular purification scheme. Using the akta prime plus october 22, 2012 3 j e n n i f e r a r r u d a attaching the column and washing the ethanol from the column. Cytiva formerly ge healthcare life sciences 8,742 views 2. Ge healthcare akta purifier 10 fplc system youtube. Never place the liquids below the pump unless the user manual states that it is. Mar 05, 2020 akta purifier manual pdf about this manual. Download ge akta pure operating instructions manual. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse.